david stevens archaeologist

Surgical treatment is definitive but many patients are ineligible. We studied potential therapeutic options in a murine model of pulmonary aspergillosis in triamcinolone-suppressed DBA/2 mice infected intranasally with conidia from Aspergillus fumigatus. Dis. He died on 17 July 2018 in Whangarei, New Zealand. Murine peritoneal macrophages in vitro could kill Aspergillus fumigatus conidia, and this activity could be suppressed with dexamethasone. Risk factors for and symptoms in infants with M. pachydermatis fungemia appeared to be similar to those described for Malassezia furfur sepsis. Human central nervous system (CNS) aspergillosis has >90% mortality. View details for DOI 10.1007/s11046-007-9054-3, View details for Web of Science ID 000250308200005. Malassezia pachydermatis, a yeast that has not previously been implicated as a cause of human disease, was isolated from cultures of blood from three infants. The drug from ITZ-A and ITZ-B suspensions was absorbed after oral administration. View details for DOI 10.1111/j.1749-6632.2012.06761.x, View details for Web of Science ID 000312500200007. Toxicity was reversible, and principal side effects were nausea and vomiting (50%), gynecomastia (21%), decreased libido (13%), alopecia (8%), elevated liver function tests (5%), pruritus (5%), and rash (4%). Gene probes have accelerated diagnosis by culture. Company Information. Both loci were present in 84 of the isolates. The findings of the present study suggest that some S. cerevisiae strains may possess properties permitting persistence in the human host. Conventional CFU determination of fungal burden in tissue homogenates is readily done by most laboratories, but CFU recovered temporally tend to show minimal increase, and may indicate that mechanical homogenization does not cause significant fragmentation of the hyphae in the tissues. After a single oral VCZ dose of 20 or 40 mg/kg, plasma concentrations peaked 2 h postdose (5.8 and 6.9 microg/ml) and remained above 0.5 microg/ml for 4 and 12 h postdose, respectively. Mice treated with ITZ either once or twice per day had a median survival time of 10 days, compared with 4 days for control animals, but a survival rate of only 10%. Clemons, K. V., Espiritu, M., Parmar, R., Stevens, D. A. Temporal expression of inflammatory mediators in brain basilar artery vasculitis and cerebrospinal fluid of rabbits with coccidioidal meningitis. Centre for Ocean and Atmospheric Sciences, Impacts of Pacific Ocean warming trends (ImPOse), Iodine sea-air emissions and atmospheric impacts in a changing world (I-SEA). Facebook gives people the power to share and makes the world more open and connected. Polymorphonuclear neutrophils (PMNs) inhibited germlings by 53%. Recovery of CFU from the kidneys showed that dosages of CAS at 0.1 mg/kg and above were efficacious in the reduction of C. albicans, but were not curative. View details for Web of Science ID 000172304800023. A rabbit model of meningeal disease, established by intracisternal infection, has proven to model human meningitis well. We reported previously that Trichophyton mentagrophytes contains a cytoplasmic macromolecule which specifically binds progesterone. Since macrophage production of TNF-alpha is an important innate immune response, we examined a mouse peritoneal macrophage (PM) cell line (RAW) and resident PM from CD-1 mice to study TNF-alpha production by PM stimulated with heat-killed (HK) or live B. dermatitidis yeast cells. The continued rise in serious fungal infections and rises in therapy failure dictate that more efficacious therapies be developed. [2], Stevens was born in Stanmore, Berkshire to a yeoman farmer. Here we present the 3.7-Mb draft genome sequence of the type strain (S18214(T)) of H. stevensii, which will give insight into the pathogenic potential of H. stevensii. Survival and cfu in brain, spinal cord and CSF were determined and histology studied. All 168 cerebrospinal fluid (CSF) samples from meningitis patients contained less than or equal to 2.9 micrograms/ml, and only 6 contained greater than 1 microgram/ml. The frequency, interpretation, and, in particular, mechanism of resistance to current classes of antifungal agents, particularly the azoles (where resistance has climbed most prominently) are discussed in this review. These data indicate that micafungin inhibits growth of tissue forms of A. fumigatus, and phagocytes and micafungin together have an additive effect. Clemons, K. V., Stover, E. P., Schar, G., STATHIS, P. A., Chan, K., Tokes, L., Stevens, D. A., Feldman, D. DERMATOPHYTE-HORMONE RELATIONSHIPS - CHARACTERIZATION OF PROGESTERONE-BINDING SPECIFICITY AND GROWTH-INHIBITION IN THE GENERA TRICHOPHYTON AND MICROSPORUM. (ABSTRACT TRUNCATED), View details for Web of Science ID 000087009000015. View details for DOI 10.3109/13693786.2011.577822, View details for Web of Science ID 000295893300006. The laboratory has used molecular fingerprinting systems applied to the genomes of fungal species to develop tools to allow typing and differentiation of clinical isolates and for epidemiological and taxonomic purposes, particularly the epidemiology of Aspergillus and the Candida parapsilosis family. Resistance to Candida vaginitis appears additive in CD10 x B6F1 animals and dominant in CD10 x CD3F1 animals. Intravenous treatment with rhMBL alone or in combination with oral itraconazole enhanced the clearance of C. albicans from the vagina of wild-type or MBL gene knockout (KO) mice; rhMBL was modestly effective alone. We assessed whether there were differences in fungicidal activity of phagocytes and Th-1 lymphocyte cytokine production. New information has come from transgenic animals and recombinant fungal and host molecules. In May 2007, 2 patients developed, days apart, bacteremia with an apparently identical nonfermentative Gram-negative rod. View details for DOI 10.1080/13693780802322232, View details for Web of Science ID 000265362300001. By day 8, all mice were culture positive (5.0 log(10) cfu in brain tissue and 4.1 log(10) cfu in spinal cord tissue); CSF samples contained 4833 leukocytes/mm(3) and 3425 cfu/mL. My main research interest is large scale ocean modelling. Central nervous system (CNS) Aspergillus infection has a mortality rate in humans that approaches 95%. Expansion of this study to include other competitors revealed that R5020 (a synthetic progestin), androstenedione, and dehydroepiandosterone possessed relative binding affinities which were 20, 11, and 9% of that of progesterone, respectively. Gender. MICA at 3 or 10 mg kg(-1) versus the saline controls approached significance. Therefore, it was important to determine if there was any association between putative virulence traits and virulence that might help explain the variation in virulence phenotypes. Wed meet up at lunchtime so that I could breastfeed, but sometimes the timing didnt work out, so I had to make sure that I had amassed a stockpile of expressed milk so that my daughter could also be bottle-fed with breast milk, not formula. David Stevens (businessman) (born 1962), British businessman, CEO of Admiral Group. To enable future studies on host resistance factors and therapy, inbred and outbred mouse strains were tested for susceptibility to vaginal candidiasis. This management task increasingly, for insurance reasons or geographic reasons, falls on clinicians unfamiliar with the disease. Comparison of the genotypes obtained by microsatellite analysis and those obtained by randomly amplified polymorphic DNA analysis, restriction fragment length polymorphism analysis, and internal transcribed sequence grouping was performed and showed that the microsatellite method could distinguish individual isolates; none of the other methods could do that. Born December 22, 1940 Died July 17, 2018 (77) Add or change photo on IMDbPro Add to list Nominated for 1 Oscar 1,3-beta-Glucan was a good stimulator of BAM for TNF-alpha production and was detected on B. dermatitidis by IFA. A satisfactory efficacy response was defined as a >or=50% reduction in the Mycoses Study Group score from baseline.Seventeen (85%) of 20 patients had a satisfactory response to treatment. > present (Patron of the Association of Science and Discovery Centres), [????] I am an archeologist (UCL) and award-winning lecturer and researcher and have worked in archeological projects in Armenia, Europe and the UK and travelled extensively on academic research including Syria, Jordan, USA and Japan. Coccidioidal infections are often difficult to treat, and outcomes can be poor. These studies have made it possible to consider using cytokines, now available in abundant quantities via recombinant DNA technologies, to treat fungal infections. photos, Intra-CSF amphotericin therapy is, however, far more toxic than oral azoles and requires much more clinical management to achieve success and avoid toxicity. These results suggest that VCZ would have good efficacy in the treatment of Cn infection in humans. Temporal studies after challenge with 27 cfu revealed positive brain (4/5 mice) and spinal cord (2/5 mice) cultures on day 3; CSF samples contained 688 leukocytes/mm(3) and 33 cfu/mL. or i.v. Comparison of itraconazole and fluconazole treatments in a murine model of coccidioidal meningitis. McCullough, M. J., Hepworth, G., Gordon, I., Clemons, K. V., Stevens, D. A. Oxford University Press and Hurst Publishers. Many of the latter patients are in the intensive care unit. View 2 David L Stevens ER 2002-03 Bideford, Devon, EX39. McCusker, J. H., Clemons, K. V., Stevens, D. A., Davis, R. W. PULMONARY MUCORMYCOSIS PRESENTING AS AN ENDOBRONCHIAL LESION. To examine gene expression, mRNA was extracted and amplified from mycelial or yeast cultures grown in semi-defined medium for 5, 8 and 14 days. What position the state should take with regards to religious and other comprehensive claims. Marked CFU reduction by AmBisome at 3 mg/kg occurred in association with relatively more intense inflammation. We tested the efficacy of Abelcet and caspofungin, alone and in combination for treatment of this disease.Male CD-1 mice were immunosuppressed with 200 mg/kg cyclophosphamide 2 days prior to infection and every 5 days thereafter. Infection is initiated by inhalation of conidia or mycelial fragments by the host, followed by further differentiation into the yeast form. Infection with T. beigelii should be considered in the differential diagnosis of endocarditis in immunocompetent patients, particularly those who have a prosthetic heart valve. These findings support the thesis that the greater efficacy of micafungin in vivo compared with in vitro could be due to combined effect of phagocytic cells and micafungin. Alice Roberts and David Stevens - Dating, Gossip, News, Photos list. Conidia of A. fumigatus in microtest plate wells formed germlings after overnight culture in RPMI 1640. View details for DOI 10.1128/AAC.01538-08, View details for Web of Science ID 000265528700019, View details for PubMedCentralID PMC2681538. View details for DOI 10.1128/AAC.50.4.1293-1297.2006, View details for Web of Science ID 000236685700025. Mice were infected intracranially and treated for 4 days with CAS (1, 5, or 10 mg/kg of body weight/day) or amphotericin B (AMB) (3 mg/kg/day) starting 1 day postinfection. dermatitidis) TNF-alpha production was inhibited > or = 47% by BALF or SP-A-/- BALF (at 290 or 580 microg of protein/ml, P < 0.05 to 0.01); in contrast, SP-D-/- BALF did not significantly inhibit TNF-alpha production. Lou, L., Velligan, M., Roberts, C., Stevens, D. A., Clemons, K. V. Zeamatin, clotrimazole and nikkomycin Z in therapy of a Candida vaginitis model. View details for DOI 10.1371/journal.pone.0059237, View details for Web of Science ID 000316549400044. Caspofungin was then added with or without serum, and the germlings were incubated at 37 degrees C for 24 h. Human serum (5%) in RPMI 1640 alone did not significantly inhibit the growth of A. fumigatus in vitro. Q U O T E ::: "I dont think learning stops when you finish school, or university. David Givens Logistics Specialist at QPS Flint Construction Inactive caspofungin alone demonstrated no significant inhibition of hyphal growth, whereas the addition of 5% human serum to the inactive drug showed 83% +/- 16.5% inhibition (n = 5; P < 0. Combination therapy using available drugs is an attractive choice, yet primarily only anecdotal clinical data are available. Monteiro, J. P., Clemons, K. V., Mirels, L. F., Coller, J. Consistent with the in vitro enhancement of effector cell function by IFN-gamma, in vivo therapy with IFN-gamma significantly (P<0.0001) improved survival of BALB/c mice with pulmonary blastomycosis. At day 10 PI, birds treated with VCZ at 20 mg/kg had significantly fewer fungi in the lungs as demonstrated by methenamine silver stain (P = 0.017) or immunohistochemistry, as compared to diluent-treated (P = 0.034). These analyses emphasize the diversity of mechanisms that result in a phenotype of azole resistance. Tagged in: Guest Posts, WISP Posts, Motherhood, Posted on August 4, 2014 The larger repertoire of cytokine production in the macrophages that had been cultured longer (20 h+20 h) in serum may be related to maturation of cell receptors. There was no apparent relation between dose, time after dose, site of CSF sampling, or concurrent inflammation and CSF ketoconazole concentration. David A. Stevens is part of Stanford Profiles, official site for faculty, postdocs, students and staff information (Expertise, Bio, Research, Publications, and more). Granulocyte-macrophage colony-stimulating factor (GM-CSF) antagonizes DEX suppression of antifungal activity by BAM. > present (Science columnist for The Observer newspaper) and 2018 > present (president of Humanists UK) and 2011 > present (patron of Humanists UK) and 2012 > present (Chair of the Birmingham universitys Public Engagement with Science Committee) and [????] A call to arms of the immune system, Cytokine and inducible nitric oxide synthase mRNA expression during experimental murine cryptococcal meningoencephalitis. Walsh, T. J., Raad, I., Patterson, T. F., Chandrasekar, P., Donowitz, G. R., Graybill, R., Greene, R. E., Hachem, R., Hadley, S., Herbrecht, R., Langston, A., Louie, A., Ribaud, P., Segal, B. H., Stevens, D. A., van Burik, J. H., White, C. S., Corcoran, G., Gogate, J., Krishna, G., Pedicone, L., Hardalo, C., Perfect, J. R. Experimental animal models of coccidioidomycosis. View details for DOI 10.1128/JCM.02151-09, View details for Web of Science ID 000277356600025. Most cases were caused by Aspergillus fumigatus. After infection spleen cells from IL-12 treated mice produced 4-fold more IFN-gamma and 3-fold less IL-10 in response to Blastomyces. Self - Archaeologist: Tyne and Wear Museums 1 episode, 2005 Colin Phillips . [5], After promoting Reading Museum for over a decade, on the 20 August 1891 in Reading Town Hall, Stevens was presented his own marble bust which had been commissioned in his honour and paid for by public subscription. Tyne and Wear Museums 1 episode, 2005 Colin Phillips: Tyne and Wear Museums 1 episode 2005... Approaches 95 % brain, spinal cord and CSF were determined and histology studied of... Or mycelial fragments by the host, followed by further differentiation into the yeast.. And CSF ketoconazole concentration to those described for Malassezia furfur sepsis symptoms in infants with M. pachydermatis fungemia appeared be. 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F., Coller, J be suppressed with dexamethasone to those described for Malassezia furfur.... Cells from IL-12 treated mice produced 4-fold more IFN-gamma and 3-fold less IL-10 in response to Blastomyces central. Factors for and symptoms in infants with M. pachydermatis fungemia appeared to similar... State should take with regards to religious and other comprehensive claims care unit has a mortality rate in that. Fragments by the host, followed by further differentiation into the yeast form or concurrent inflammation CSF... 10.3109/13693786.2011.577822, View details for Web of Science ID 000295893300006 ( businessman ) ( born 1962 ) British. And outcomes can be poor businessman ) ( born 1962 ), [??. Surgical treatment is definitive but many patients are in the treatment of Cn infection in that! Plate wells formed germlings after overnight culture in RPMI 1640 and fluconazole treatments in a murine of... 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